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Can I read Quantitative Imaging of Single-cell Phenotypes in Cancer Cells Cultured on Hydrogel Surfaces on EtoBox?
Quantitative Imaging of Single-cell Phenotypes in Cancer Cells Cultured on Hydrogel Surfaces by Vicky Bousgouni; Chris Bakal is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Quantitative Imaging of Single-cell Phenotypes in Cancer Cells Cultured on Hydrogel Surfaces about?
## Summary Small interfering RNA (siRNA) screening approaches used with quantitative single-cell analysis can uncover the roles of genes in cell morphogenesis. Here, we present a high-throughput automated phenotypic screening technique to quantify a single cell shape in cancer cells cultured on top of soft 3D hydrogels. We describe reverse transfection of cells with siRNAs and seeding of these cells on top of collagen, followed by image analysis to quantify morphology of a single cell and population levels in low-elasticity matrices. For complete details on the use and execution of this protocol, please refer to Bousgouni et al. (2022).^1^
Who reads Quantitative Imaging of Single-cell Phenotypes in Cancer Cells Cultured on Hydrogel Surfaces?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Vicky Bousgouni; Chris Bakal
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)