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The effect of nitrogen dioxide exposure on the release of surfactant isolated from neonatal rabbit type II pneumocytes in culture by Matthew Knight; Madhavi Sekharam; Jay Patel is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Nitrogen dioxide (NO~2~) is a well‐known environmental air toxin, produced from a variety of sources, including cigarette smoke. Because of the growing knowledge of the harmful effects of passive smoking on children, we decided to study the effect of NO~2~ exposure on the release of surfactant from isolated neonatal type II pulmonary epithelial cells. After isolation from 1 to 4 day old rabbits, type II epithelial cells were allowed to adhere for 18 hours, washed, media changed, and were exposed to either 5% CO~2~ in room air or NO~2~, 5 ppm, for 2 hours (all results mean ± sd; comparisons, paired __t__‐test). There was no difference in cell number or viability prior to exposure. Cells exposed to NO~2~ had an increase in LDH release [LDH activity in media/(LDH in media + cells) x 100], air 12.6 ± 2.2%, NO~2~ 21.7 ± 3.7%, (__p__ < 0.05). NO~2~‐exposed cells also had an increase in total phospholipid (μg/cell culture dish) in media compared to air exposed, air 170.13 ± 7.54, NO~2~ 195.15 ± 11.2, (__p__ < 0.05). ^3^H‐choline incorporation as a precursor to disaturated phosphati‐dylcholine (DSPC) was also conducted during exposure to either air or NO~2~. Incorporation of ^3

Who reads The effect of nitrogen dioxide exposure on the release of surfactant isolated from neonatal rabbit type II pneumocytes in culture?

Author

Matthew Knight; Madhavi Sekharam; Jay Patel

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); VCH Publishers; Wiley (ISSN 0887-2082)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)