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Can I read Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing on EtoBox?
Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing by Israel Alshanski; Suraj Toraskar; Ariel Shitrit; Daniel Gordon-Levitan; Prashant Jain; Raghavendra Kikkeri; Mattan Hurevich; Shlomo Yitzchaik is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing about?
Sialic acid recognition and hydrolysis are essential parts of cellular function and pathogen infectivity. Neuraminidases are enzymes that detach sialic acid from sialosides, and their inhibition is a prime target for viral infection treatment. The connectivity and type of sialic acid influence the recognition and hydrolysis activity of the many different neuraminidases. The common strategies to evaluate neuraminidase activity, recognition, and inhibition rely on extensive labeling and require a large amount of sialylated glycans. The above limitations make the effort of finding viral inhibitors extremely difficult. We used synthetic sialylated glycans and developed a label-free electrochemical method to show that sialoside structural features lead to selective neuraminidase biosensing. We compared Neu5Ac to Neu5Gc sialosides to evaluate the organism-dependent neuraminidase selectivity–sensitivity relationship. We demonstrated that the type of surface and the glycan monolayer density direct the response to either binding or enzymatic activity. We proved that while the hydrophobic glassy carbon surface increases the interaction with the enzyme hydrophobic interface, the negatively ch
Who reads Biocatalysis versus Molecular Recognition in Sialoside-Selective Neuraminidase Biosensing?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Israel Alshanski; Suraj Toraskar; Ariel Shitrit; Daniel Gordon-Levitan; Prashant Jain; Raghavendra Kikkeri; Mattan Hurevich; Shlomo Yitzchaik
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)