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Oxidation of Bovine Serum Albumin: Identification of Oxidation Products and Structural Modifications by Sofia Guedes; Rui Vitorino; Rosário Domingues; Francisco Amado; Pedro Domingues is a Chemistry article available to read on EtoBox.

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## Abstract Albumin is an important plasma antioxidant protein, contributing to protecting mechanisms of cellular and regulatory long‐lived proteins. The metal‐catalyzed oxidation (MCO) of proteins plays an important role during oxidative stress. In this study, we examine the oxidative modification of albumin using an MCO __in vitro__ system. Mass spectrometry, combined with off‐line nano‐liquid chromatography, was used to identify modifications in amino acid residues. We have found 106 different residues oxidatively damaged, being the main oxidized residues lysines, cysteines, arginines, prolines, histidines and tyrosines. Besides protein hydroxyl derivatives and oxygen additions, we detected other modifications such as deamidations, carbamylations and specific amino acid oxidative modifications. The oxidative damage preferentially affects particular subdomains of the protein at different time‐points. Results suggest the oxidative damage occurs first in exposed regions near cysteine disulfide bridges with residues like methionine, tryptophan, lysine, arginine, tyrosine and proline appearing as oxidatively modified. The damage extended afterwards with further oxidation of cysteine

Who reads Oxidation of Bovine Serum Albumin: Identification of Oxidation Products and Structural Modifications?

Author

Sofia Guedes; Rui Vitorino; Rosário Domingues; Francisco Amado; Pedro Domingues

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)