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Lactic Acid Production from Mussel Processing Wastes with an Amylolytic Bacterial Strain by J Pintado; J.P Guyot; M Raimbault is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The aim of this work was to study the capability of amylolytic lactic acid bacteria to hydrolyse the glycogen in ( ) mussel processing wastes MPW for lactic acid production. From four strains tested, Lactobacillus plantarum A6 was selected. Cultures of this strain on MRS medium with starch or mussel glycogen, showed similar le ̈els of amylase production for both polysaccharides. With glycogen, sugar consumption rate was lower than with soluble starch and, although the micro-organism produced higher yields of enzyme, a fraction of approximately 20% of the total sugars remained undegraded. Howe ̈er, the different substrates did not seem to induce the production of different types of amylase and the ̈alues of the apparent K for starch and for glycogen were m close, re ̈ealing a similar affinity for both substrates. Therefore, the differences in hydrolysis and consumption of the substrate seem to be related with the higher grade of branching of glycogen. Culture in MPW showed a decrease in pH unfa ̈ourable for amylolytic acti ̈ity and uncoupling between lactic acid and biomass production. By controlling pH and increasing the initial protein concentration to a ̈oid uncoupling, similar

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Author

J Pintado; J.P Guyot; M Raimbault

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)