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A Single-Laboratory Validation of a UPLC Method for Determination of Folic Acid in Various Dietary Supplement Dosage Forms by Lusi A; Mina Fakhary; Niloufar Rahimi Gaeini; Jennifer M Solano; Mohamed Koroma is a Chemistry article available to read on EtoBox.
What is A Single-Laboratory Validation of a UPLC Method for Determination of Folic Acid in Various Dietary Supplement Dosage Forms about?
## Abstract ## Background Folic acid is an essential nutrient necessary for the synthesis of nucleic acids (DNA and RNA) and certain amino acids. There are no scientifically validated analytical methods for folic acid applicable to all dosage forms. ## Objective A single-laboratory method was validated for the determination of folic acid content in various dietary supplement dosage forms. This method used ultra-performance liquid chromatography/diode-array detector (UPLC/PDA) to determine the folic acid content in dietary supplements in the form of tablets, two-piece capsules, powder drinks, softgels, and gummies. ## Method The ultra-performance liquid chromatography/diode-array detector method was evaluated for linearity, limit of detection (LOD), limit of quantification (LOQ), repeatability, recovery, specificity, and system suitability. ## Results Linearity of the folic acid standard was shown to be linear in the range of 0.45 μg/mL to 7.37 μg/mL. LOD and LOQ of folic acid were 0.089 and 0.268 μg/mL, respectively. The repeatability of nine samples from five matrixes resulted in 1.15–4.82% relative standard deviation (RSD). Five samples with five different matrixes spiked with 25
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- Author
- Lusi A; Mina Fakhary; Niloufar Rahimi Gaeini; Jennifer M Solano; Mohamed Koroma
- Publisher
- Oxford University Press (OUP)
- Published
- 2023
- Language
- EN
- Field
- Chemistry (Physical Sciences)