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Cellular Distribution of 6-Phosphofructo-1-Kinase Isoenzymes in Rat Brain by U. Zeitschel; M. Bigl; K. Eschrich; V. Bigl is a Neuroscience article available to read on EtoBox.

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Abstract: In the brain, all three isoenzyme types [muscle (M), liver (L), and brain (C)] of 6‐phosphofructo‐1‐kinase (PFK; EC 2.7.1.11) occur, forming a complex mixture of homo‐ and heterotetramers. The PFK isoenzyme pattern of the different brain cell types is yet unknown. In the present study, we investigated the distribution of the PFK isoenzyme subunits in primary and secondary cell cultures and in bulk‐isolated cells of rat brain by means of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and western blotting. All three PFK isoenzymes are present in all cell types but in different proportions. The cellular distribution of the PFK isoenzymes in situ was studied immunohistochemically with different polyclonal antisera against purified rat PFKs. The monospecific antibody against M‐type PFK stained preferentially the perinuclear areas of neurons and glial cells. The antibodies that in immunoblots detected mainly the L‐type PFK showed a characteristic staining in only the cytoplasma and the processes of cells, whereas the C‐type antibodies almost homogeneously stained whole cell bodies as well as large dendrites. Because the PFK isoenzymes differ with respect to their all

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Author

U. Zeitschel; M. Bigl; K. Eschrich; V. Bigl

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1996

Language

EN

Field

Neuroscience (Life Sciences)