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Modulation of the pharmacokinetics of macromolecular contrast material by avidin chase: MRI, optical, and inductively coupled plasma mass spectrometry tracking of triply labeled albumin by Hagit Dafni; Assaf Gilead; Nava Nevo; Raya Eilam; Alon Harmelin; Michal Neeman is a Medicine article available to read on EtoBox.

## Abstract The goal of this work was to develop an MRI method for mapping the clearance of interstitial macromolecular plasma proteins after their extravasation from permeable blood vessels. To that end, a well‐defined window of exposure to elevated blood levels was generated by inducing rapid clearance of macromolecular contrast material from the blood. Experimental removal of the intravascular component allowed subsequent tracking of clearance from the interstitial compartment in the absence of further contrast extravasation. The contrast material was based on albumin triply labeled with biotin, fluorescent tag, and GdDTPA, allowing optical, inductively coupled plasma mass spectrometry (ICP‐MS) and MRI detection. The biotin tag was used here for in vivo chasing of the contrast material from the blood by intravenous administration of avidin. Upon administration of avidin the contrast material disappeared from the blood vessels and was cleared by the liver and spleen as detected by MRI, fluorescence of blood samples and histological sections, and by ICP‐MS. Nonbiotinylated fluorescent albumin was not affected by administration of avidin. Contrast material that extravasated from le

Author

Hagit Dafni; Assaf Gilead; Nava Nevo; Raya Eilam; Alon Harmelin; Michal Neeman

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0740-3194)

Published

2003

Language

EN

Field

Medicine (Health Sciences)