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Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion by Grant M Sumida; Tyler M Tomita; Wenting Shih; Soichiro Yamada is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion about?

## Background Maintaining proper adhesion between neighboring cells depends on the ability of cells to mechanically respond to tension at cell-cell junctions through the actin cytoskeleton. Thus, identifying the molecules involved in responding to cell tension would provide insight into the maintenance, regulation, and breakdown of cell-cell junctions during various biological processes. Vinculin, an actin-binding protein that associates with the cadherin complex, is recruited to cell-cell contacts under increased tension in a myosin II-dependent manner. However, the precise role of vinculin at force-bearing cell-cell junctions and how myosin II activity alters the recruitment of vinculin at quiescent cell-cell contacts have not been demonstrated. ## Results We generated vinculin knockdown cells using shRNA specific to vinculin and MDCK epithelial cells. These vinculin-deficient MDCK cells form smaller cell clusters in a suspension than wild-type cells. In wound healing assays, GFP-vinculin accumulated at cell-cell junctions along the wound edge while vinculin-deficient cells displayed a slower wound closure rate compared to vinculin-expressing cells. In the presence of blebbistati

Who reads Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion?

Author

Grant M Sumida; Tyler M Tomita; Wenting Shih; Soichiro Yamada

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)