Can I read Reversible unfolding of bovine odorant binding protein induced by guanidinium hydrochloride at neutral pH on EtoBox?

Reversible unfolding of bovine odorant binding protein induced by guanidinium hydrochloride at neutral pH by Alberto Mazzini; Alessia Maia; Mariella Parisi; Robert Tibor Sorbi; Roberto Ramoni; Stefano Grolli; Roberto Favilla is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Reversible unfolding of bovine odorant binding protein induced by guanidinium hydrochloride at neutral pH about?

An analysis of the unfolding and refolding curves at equilibrium of dimeric bovine odorant binding protein (bOBP) has been performed. Unfolding induced by guanidinium chloride (GdnHCl) is completely reversible as far as structure and ligand binding capacity are concerned. The transition curves, as obtained by fluorescence and ellipticity measurements, are very similar and have the same protein concentration-independent midpoint (C1/2 approximately 2.6 M). This result implies a sequential, rather than a concerted, unfolding mechanism, with the involvement of an intermediate. However, since it has not been detected, this intermediate must be present in small amounts or have the same optical properties of either native or denatured protein. The thermodynamic best fit parameters, obtained according to a simple two-state model, are: deltaG degrees un,w = 5.0 +/- 0.6 kcal mol(-1), m = 1.9 +/- 0.2 kcal mol(-1) M(-1) and C1/2 = 2.6 +/- 0.1 M. The presence of the ligand dihydromyrcenol has a stabilising effect against unfolding by GdnHCl, with an extrapolated deltaG degrees un,w of 22.2 +/- 0.9 kcal mol(-1), a cooperative index of 3.2 +/- 0.3 and a midpoint of 4.6 +/- 0.4 M. The refolding c

Who reads Reversible unfolding of bovine odorant binding protein induced by guanidinium hydrochloride at neutral pH?

Author

Alberto Mazzini; Alessia Maia; Mariella Parisi; Robert Tibor Sorbi; Roberto Ramoni; Stefano Grolli; Roberto Favilla

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1570-9639)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)