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Tanshinol suppresses osteosarcoma by specifically inducing apoptosis of U2-OS cells through p53-mediated mechanism 1 1 Introduction 1 2 Materials and methods 2 2.1 Chemicals and reagents 2 2.2 Cell lines and culture 2 2.3 Zebrafish and handling 2 2.4 Xenograft animal assay 2 2.5 CCK-8 cell viability assay 2 2.6 Cell morphology and DAPI staining 2 2.7 Immunofluorescence 3 2.8 Flow cytometry 3 2.9 RNA extraction and quantitative real-time PCR (qPCR) assay 3 2.10 Western blot (WB) analysis 3 2.11 Statistical analysis 3 3 Results 3 3.1 Anti-OS effect of TS in vivo 3 3.2 TS inhibited cell viability of U2-OS cells 3 3.3 TS induced apoptosis of U2-OS cells 6 3.4 TS-regulated pro-apoptotic genes expression in U2-OS cells 7 3.5 TS-regulated protein expression in U2-OS cells 8 4 Discussion 8 5 Conclusion 9 Author contributions 9 Funding 9 Declaration of competing interest 9 Appendix A Supplementary data 9 References 9
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Common subject areas: history, science, philosophy, social sciences.
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- nonfiction