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Activation of mRNA translation by phage protein and low temperature: the case of Lactococcus lactis abortive infection system AbiD1 by Elena Bidnenko; Alain Chopin; S Dusko Ehrlich; Marie-Christine Chopin is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Activation of mRNA translation by phage protein and low temperature: the case of Lactococcus lactis abortive infection system AbiD1 about?

Background: Abortive infection (Abi) mechanisms comprise numerous strategies developed by bacteria to avoid being killed by bacteriophage (phage). Escherichia coli Abis are considered as mediators of programmed cell death, which is induced by infecting phage. Abis were also proposed to be stress response elements, but no environmental activation signals have yet been identified. Abis are widespread in Lactococcus lactis, but regulation of their expression remains an open question. We previously showed that development of AbiD1 abortive infection against phage bIL66 depends on orf1, which is expressed in mid-infection. However, molecular basis for this activation remains unclear. ## Results: In non-infected AbiD1+ cells, specific abiD1 mRNA is unstable and present in low amounts. It does not increase during abortive infection of sensitive phage. Protein synthesis directed by the abiD1 translation initiation region is also inefficient. The presence of the phage orf1 gene, but not its mutant AbiD1 R allele, strongly increases abiD1 translation efficiency. Interestingly, cell growth at low temperature also activates translation of abiD1 mRNA and consequently the AbiD1 phenotype, and oc

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Author

Elena Bidnenko; Alain Chopin; S Dusko Ehrlich; Marie-Christine Chopin

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-; Springer Science and Business Media LLC (ISSN 1471-2199)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)