Opening book details…
Can I read CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination on EtoBox?
CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination by Zhengyan Weng; Zheng You; Huijie Li; Guangfu Wu; Yang Song; He Sun; Ava Fradlin; Camille Neal-Harris; Mengshi Lin; Xue Gao; Yi Zhang is a Engineering article available to read on EtoBox.
What is CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination about?
Quantitative polymerase chain reaction as a powerful tool for DNA detection has been pivotal to a vast range of applications, including disease screening, food safety assessment, environmental monitoring, and many others. However, the essential target amplification step in combination with fluorescence readout poses a significant challenge to rapid and streamlined analysis. The discovery and engineering of the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) technology have recently paved the way for a novel approach to nucleic acid detection, but the majority of current CRISPR-mediated DNA detection platforms are limited by insufficient sensitivity and still require target preamplification. Herein, we report a CRISPR-Cas12a-mediated graphene field-effect transistor (gFET) array, named CRISPR Cas12a-gFET, for amplification-free, ultrasensitive, and reliable detection of both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) targets. CRISPR Cas12a-gFET leverages the multiturnover trans-cleavage activity of CRISPR Cas12a for intrinsic signal amplification and ultrasensitivity of gFET. As demonstrated, CRISPR Cas12a-gFET achieves
Who reads CRISPR-Cas12a Biosensor Array for Ultrasensitive Detection of Unamplified DNA with Single-Nucleotide Polymorphic Discrimination?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Zhengyan Weng; Zheng You; Huijie Li; Guangfu Wu; Yang Song; He Sun; Ava Fradlin; Camille Neal-Harris; Mengshi Lin; Xue Gao; Yi Zhang
- Publisher
- American Chemical Society (ACS)
- Published
- 2023
- Language
- EN
- Field
- Engineering (Physical Sciences)