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A Method for Quantitative Determination of Deuterium Content in Biological Material by Yihui He; Caihua Tang; Jiangsen Mao; Ziyang Liu; Weixing Huang; Zi'an Mao is a Chemistry article available to read on EtoBox.

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## Abstract A method was developed for quantitative determination of deuterium incorporated into live organisms or biological macromolecules. The deuterated biological material was mixed with a bovine serum albumin (BSA) supporter to make a homogeneous sample for which the __δD__ value (vs. VSMOW) was analyzed using a dual‐inlet gas isotope mass spectrometer. The method is described in detail, and the equation for calculation of deuterium content is presented, i.e., __C__ = 1/500 × __k__ × __R__~__VSMOW__~ × __C__ × 10^6^ ppm. Deuterated hepatitis A virus (HAV) RNA and BSA were systematically investigated. The results demonstrate that the method is capable of direct measurement of deuterium content, and is highly repeatable and reliable with a standard deviation of ±3‰. It is stressed that the quantity of deuterated sample required is extremely small as a result of using BSA as supporter. The method may be applied in many fields, and has the strengths of simplicity, relative cheapness, and robustness. Copyright © 2005 John Wiley & Sons, Ltd.

Who reads A Method for Quantitative Determination of Deuterium Content in Biological Material?

Author

Yihui He; Caihua Tang; Jiangsen Mao; Ziyang Liu; Weixing Huang; Zi'an Mao

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2005

Language

EN

Field

Chemistry (Physical Sciences)