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Flow Cytometric Analysis of Whole Blood Lysis, Three Anticoagulants, and Five Cell Preparations by Patricia H. Carter; Sandra Resto‐Ruiz; Glennelle C. Washington; Steve Ethridge; Alessio Palini; Robert Vogt; Myron Waxdal; Thomas Fleisher; Philip D. Noguchi; Gerald. E. Marti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Flow Cytometric Analysis of Whole Blood Lysis, Three Anticoagulants, and Five Cell Preparations about?
## Abstract We studied the effects of anticoagulants and cell preparation methods on lymphocyte forward‐angle s catter (FSC), autofluorescence, and immunofluorescent staining for CD45, CD14, and CD13. Blood samples collected in ethylenediaminetetracetic acid (EDTA), heparin, and acid citrate dextrose (ACD) were processed by using conventional Hypaque‐Ficoll (HF) separation and four whole blood (WR) lysis techniques: Immuno‐lyse, Q‐Prep, FACS Lyse, and Gen Trak Lysis. Lymphocytes prepared by using three of the four whole blood methods gave FCS values comparable to those isolated by HF, while one method (FACS Lyse) gave consistently lover values. Autofluorescence values were comparable by all methods except Immuno‐lyse, which showed consistently higher values in blood stored for 24 h with any anticoagulant. Immunofluorescent values for CD45‐stained cells were quite consistent across all methods, and among the whole blood methods, FACS Lyse and Q‐Prep uniformly gave the highest purity of CD45‐positive cells in the lymphocyte light scatter gates. Additionally, propidium iodide (PI) analyses of CD45‐stained whole blood, and analyzed without lysis, confirmed that ACD and heparin were sup
Who reads Flow Cytometric Analysis of Whole Blood Lysis, Three Anticoagulants, and Five Cell Preparations?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Patricia H. Carter; Sandra Resto‐Ruiz; Glennelle C. Washington; Steve Ethridge; Alessio Palini; Robert Vogt; Myron Waxdal; Thomas Fleisher; Philip D. Noguchi; Gerald. E. Marti
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)
- Published
- 1992
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)