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This document describes a laboratory experiment to separate and identify pigments such as chlorophylls, carotenes, and xanthophylls in spinach through extraction and thin-layer chromatography. Students extracted the compounds from spinach using methanol and petroleum ether, and then separated the pigments on the chromatography plate using a solvent of hexane, acetone, and chloroform. Several pigments were observed such as chlorophylls a and b, β-carotene, and derivatives.

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