Can I read B Cell Activating Factor Regulates Periodontitis Development by Suppressing Inflammatory Responses in Macrophages on EtoBox?

B Cell Activating Factor Regulates Periodontitis Development by Suppressing Inflammatory Responses in Macrophages by Lixia Wang; Tianyi Zhang; Zheng Zhang; Zihan Wang; Yu-Jie Zhou; Zuomin Wang is a Dentistry article available to read on EtoBox.

What is B Cell Activating Factor Regulates Periodontitis Development by Suppressing Inflammatory Responses in Macrophages about?

## Abstract ## Background B cell activating factor (BAFF) is a member of the tumor necrosis factor (TNF) superfamily with immunomodulatory effects on both innate and adaptive immune responses. Periodontitis is an inflammatory disease characterized by periodontal soft tissue inflammation and the progressive loss of periodontal ligament and alveolar bone. Macrophages are closely related to periodontitis progression. However, the role of BAFF in periodontitis development and macrophage polarization and the underlying mechanism remain unknown. ## Methods In vivo, a ligation-induced mouse model of periodontitis for BAFF blockade was established to investigate the expression of inducible nitric oxide synthase (iNOS) through real-time PCR (RT-PCR) and immunohistochemistry. In addition, the level of TNF-α in the periodontium, the number of osteoclasts, and alveolar bone resorption were observed. In vitro, RAW 264.7 macrophage cells were treated with 100 ng/mL Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) in either the presence or absence of 50 nM small interfering RNA (siRNA) targeting BAFF, followed by further incubation for 24 h. These cells and supernatants were collec

Who reads B Cell Activating Factor Regulates Periodontitis Development by Suppressing Inflammatory Responses in Macrophages?

Author

Lixia Wang; Tianyi Zhang; Zheng Zhang; Zihan Wang; Yu-Jie Zhou; Zuomin Wang

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Dentistry (Health Sciences)