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A Novel Type of Cytoplasmic Granule in Bovine Neutrophils by R Gennaro; B Dewald; U Horisberger; H U Gubler; M Baggiolini is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We obtained cell preparations containing greater than 95% neutrophils from freshly drawn bovine blood. The cells were suspended in sucrose and disrupted in a Dounce homogenizer, and the postnuclear supernate was fractionated by zonal differential sedimentation and by isopycnic equilibration. The subcellular fractions were characterized biochemically by testing for marker enzymes and other constituents known to occur in azurophil and specific granules of other species, and by electrophoretic analysis of extracts of the particulate material. In addition, each fraction was examined by random-sampling electron microscopy. We found that bovine neutrophils contain in addition to azurophil and specific granules a third type of granule, not known to occur in neutrophils of other species. These novel granules are larger, denser, and considerably more numerous than the two other types. Except for lactoferrin, they lack the characteristic constituents of azurophil granules (peroxidase, acid hydrolases, and neutral proteinases) and of specific granules (vitamin B12-binding protein). Instead, they contain a group of highly cationic proteins not found in the other granules, and they are the excl

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Author

R Gennaro; B Dewald; U Horisberger; H U Gubler; M Baggiolini

Publisher

The Rockefeller University Press

Published

1983

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)