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Enhancement of Larval RNAi Efficiency by Over-expressing Argonaute2 in Bombyx mori by Zhiqian Li; Baosheng Zeng; Lin Ling; Jun Xu; Lang You; Abu F.M. Aslam; Anjiang Tan; Yongping Huang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Enhancement of Larval RNAi Efficiency by Over-expressing Argonaute2 in Bombyx mori about?

RNA interference has been described as a powerful genetic tool for gene functional analysis and a promising approach for pest management. However, RNAi efficiency varies significantly among insect species due to distinct RNAi machineries. Lepidopteran insects include a large number of pests as well as model insects, such as the silkworm, __Bombyx mori__. However, only limited success of __in vivo__ RNAi has been reported in lepidoptera, particularly during the larval stages when the worms feed the most and do the most harm to the host plant. Enhancing the efficiency of larval RNAi in lepidoptera is urgently needed to develop RNAi-based pest management strategies. In the present study, we investigate the function of the conserved RNAi core factor, Argonaute2 (Ago2), in mediating __B. mori__ RNAi efficiency. We demonstrate that introducing __BmAgo2__ dsRNA inhibits the RNAi response in both BmN cells and embryos. Furthermore, we establish several transgenic silkworm lines to assess the roles of __BmAgo2__ in larval RNAi. Over-expressing __BmAgo2__ significantly facilitated both dsRNA-mediated larval RNAi when targeting __DsRed__ using dsRNA injection and shRNA-mediated larval RNAi wh

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Author

Zhiqian Li; Baosheng Zeng; Lin Ling; Jun Xu; Lang You; Abu F.M. Aslam; Anjiang Tan; Yongping Huang

Publisher

Ivyspring International Publisher

Published

2015

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)