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Osteoclast Generation from Osteoblasts by Kanthi Lewis is a document available to read on EtoBox.

What is Osteoclast Generation from Osteoblasts about?

The document provides instructions for generating osteoclasts from a coculture of osteoblasts and bone marrow cells. Key steps include seeding osteoblasts in media, isolating bone marrow cells from mouse femurs, seeding the bone marrow cells on the osteoblasts and adding vitamin D and PGE2, and incubating the coculture for 4-5 days with a media change after 2 days. Special requirements include using heat inactivated media for osteoblasts and non-heat inactivated media for bone marrow cells.

Author

Kanthi Lewis

Language

EN