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This document describes a spectrophotometric coupled enzyme assay to measure the activity of succinate dehydrogenase (complex II). The assay uses fumarate hydratase to convert fumarate produced by complex II to malate. Oxaloacetate decarboxylating malic enzyme then converts malate to pyruvate, producing NADPH which is detected spectrophotometrically. This provides a simple, quantitative, and specific method to measure complex II activity in isolated enzymes, membrane preparations, and tissue homogenates. Th

Author

nadia

Language

EN