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Can I read Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles on EtoBox?
Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles by Fatiha Chellat; Alexia Grandjean-Laquerriere; Richard Le Naour; Julio Fernandes; L’Hocine Yahia; Moncef Guenounou; Dominique Laurent-Maquin is a Engineering article available to read on EtoBox.
What is Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles about?
The use of nanoparticles for gene therapy is gaining more and more interest for medical applications. Chitosan is among the candidate polymers that have a potential application as a gene delivery system. Before using chitosan-DNA nanoparticles in vivo, one must study their interaction and cell's behavior. Since macrophages play an important role in inflammatory processes, this study was performed to investigate the effects of chitosan-DNA nanoparticles on human THP-1 cell line. Cytokine (TNF-alpha, IL-1beta, IL-6 and IL-10) and metalloproteinase (MMP-2 and MMP-9) release as well as their inhibitors (TIMP-1 and TIMP-2) were assessed after time course incubation with different amount of nanoparticles. Their secretion was quantified by enzyme-linked immunosorbent assay. Gelatinolytic activity of MMP-2 and MMP-9 was determined by zymography in cell supernatants and lysates. Cytokine secretion was not detected even in the presence of high amount of nanoparticles. On the contrary, the secretion of MMP-9 in cell supernatants increased significantly after 24 and 48 h in comparison with non-treated cells. MMP-2 secretion was augmented only after 48 h for the highest concentrations of nanopa
Who reads Metalloproteinase and cytokine production by THP-1 macrophages following exposure to chitosan-DNA nanoparticles?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Fatiha Chellat; Alexia Grandjean-Laquerriere; Richard Le Naour; Julio Fernandes; L’Hocine Yahia; Moncef Guenounou; Dominique Laurent-Maquin
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2005
- Language
- EN
- Field
- Engineering (Physical Sciences)