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Phosphoproteome analysis of the pathogenic bacterium Helicobacter pylori reveals over-representation of tyrosine phosphorylation and multiply phosphorylated proteins by Ruiguang Ge; Xuesong Sun; Chuanle Xiao; Xingfeng Yin; Weiran Shan; Zhuo Chen; Qing-Yu He is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Increasing evidence shows that protein phosphorylation on serine (Ser), threonine (Thr) and tyrosine (Tyr) residues is a major regulatory post‐translational modification in the bacteria. To reveal the phosphorylation state in the Gram‐negative pathogenic bacterium __Helicobacter pylori__, we carried out a global and site‐specific phosphoproteomic analysis based on TiO~2~‐phosphopeptide enrichment and high‐accuracy LC‐MS/MS determination. Eighty‐two phosphopeptides from 67 proteins were identified with 126 phosphorylation sites, among which 79 class I sites were determined to have a distribution of 42.8:38.7:18.5% for the Ser/Thr/Tyr phosphorylation, respectively. The __H. pylori__ phosphoproteome is characterized by comparably big size, high ratio of Tyr phosphorylation, high abundance of multiple phosphorylation sites in individual phosphopeptides and over‐representation of membrane proteins. An interaction network covering 28 phosphoproteins was constructed with a total of 163 proteins centering on the major __H. pylori__ virulence factor VacA, indicating that protein phosphorylation in __H. pylori__ may be delicately controlled to regulate many aspects of the metabol
Who reads Phosphoproteome analysis of the pathogenic bacterium Helicobacter pylori reveals over-representation of tyrosine phosphorylation and multiply phosphorylated proteins?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ruiguang Ge; Xuesong Sun; Chuanle Xiao; Xingfeng Yin; Weiran Shan; Zhuo Chen; Qing-Yu He
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)