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Can I read α-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells on EtoBox?
α-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells by Wook-Dong Kim; Osamu Kobayashi; Satoshi Kaneko; Yoshikiyo Sakakibara; Gwi-Gun Park; Isao Kusakabe; Hideo Tanaka; Hideyuki Kobayashi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is α-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells about?
The alpha-galactosidase from rice cell suspension cultures was purified to homogeneity by different techniques including affinity chromatography using N-epsilon-aminocaproyl-alpha-D-galactopyranosylamine as the ligand. From 11 l of culture filtrate, 28.7 mg of purified enzyme was obtained with an overall yield of 51.9%. The cDNA coding for the alpha-galactosidase was cloned and sequenced. The enzyme was found to contain 417 amino acid residues composed of a 55 amino acid signal sequence and 362 amino acid mature alpha-galactosidase; the molecular weight of the mature enzyme was thus calculated to be 39,950. Seven cysteine residues were also found but no putative N-glycosylation sites were present. The observed homology between the deduced amino acid sequences of the mature enzyme and alpha-galactosidases from coffee (Coffea arabica), guar (Cyamopsis tetragonolooba), and Mortierella vinacea alpha-galactosidase II were over 73, 72, and 45%, respectively. The enzyme displayed maximum activity at 45 degrees C when p-nitrophenyl-alpha-D-galactopyranoside was used as substrate. The rice alpha-galactosidase and Mortierella vinacea alpha-galactosidase II acted on both the terminal alpha-ga
Who reads α-Galactosidase from cultured rice (Oryza sativa L. var. Nipponbare) cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Wook-Dong Kim; Osamu Kobayashi; Satoshi Kaneko; Yoshikiyo Sakakibara; Gwi-Gun Park; Isao Kusakabe; Hideo Tanaka; Hideyuki Kobayashi
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)
- Published
- 2002
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)