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Ischemic preconditioning reduces Na+ accumulation and cell killing in isolated rat hepatocytes exposed to hypoxia by Rita Carini; Maria Grazia De Cesaris; Roberta Splendore; Marco Bagnati; Prof. Emanuele Albano is a Medicine article available to read on EtoBox.
What is Ischemic preconditioning reduces Na+ accumulation and cell killing in isolated rat hepatocytes exposed to hypoxia about?
Short periods of ischemia followed up by reperfusion are known to protect the heart against injury caused by a subsequent sustained ischemia. This phenomenon, known as ischemic preconditioning, has also been recently shown to reduce ischemic liver damage, but the mechanisms involved are still unknown. By using isolated hepatocytes as an in vitro model of liver preconditioning, we have investigated the possible effect of preconditioning on intracellular pH and Na ؉ homeostasis. Freshly isolated rat hepatocytes were preconditioned by 10 minutes of incubation under hypoxic conditions followed up by 10 minutes of reoxygenation and subsequently exposed to 90 minutes of hypoxia. Although preconditioning did not ameliorate adenosine triphosphate (ATP) depletion, preconditioned hepatocytes exhibited an increased resistance to cell killing during hypoxic incubation. Intracellular acidosis and Na ؉ accumulation developing during hypoxia were appreciably reduced in preconditioned cells. The effects of preconditioning on intracellular pH, Na ؉ homeostasis, and citotoxicity were mimicked by stimulating protein kinase C (PKC) with 4-phorbol-12myristate-13-acetate (PMA) or 1,2 dioctanoyl-glycero
Who reads Ischemic preconditioning reduces Na+ accumulation and cell killing in isolated rat hepatocytes exposed to hypoxia?
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- Author
- Rita Carini; Maria Grazia De Cesaris; Roberta Splendore; Marco Bagnati; Prof. Emanuele Albano
- Publisher
- Ovid Technologies (Wolters Kluwer Health)
- Published
- 2000
- Language
- EN
- Field
- Medicine (Health Sciences)