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Induction of Cyclooxygenase‐2 by Overexpression of the Human Catalase Gene in Cerebral Microvascular Endothelial Cells by Xiang Fang; A Steven Moore; O Joseph Nwankwo; L Neal Weintraub; W Larry Oberley; D Gary Snyder; A Arthur Spector is a Neuroscience article available to read on EtoBox.

What is Induction of Cyclooxygenase‐2 by Overexpression of the Human Catalase Gene in Cerebral Microvascular Endothelial Cells about?

Prostaglandin (PG) formation by the inducible (type 2) cyclooxygenase (COX‐2) and reactive oxygen species (ROS) have been proposed to play important roles in cerebrovascular pathological processes. To explore the relationship between ROS and COX‐2 expression, adenovirus (Ad) vectors containing cDNA for human antioxidant enzymes including catalase (Ad__Cat__), copper/zinc superoxide dismutase (Ad__Cu/ZnSOD__), and manganese superoxide dismutase (Ad__MnSOD__) were transferred into murine cerebral microvascular endothelial cells. Ad__Cat__ (100 multiplicity of infection) infection increased the content and enzymatic activity of cellular Cat threefold and decreased the intracellular peroxide level. The expression of COX‐2 mRNA and protein in cell lysates was up‐regulated, and the amount of PGE~2~ formed from exogenous arachidonic acid increased following Ad__Cat__ infection in a dose‐dependent manner, paralleling the expression of COX‐2 protein. The Ad__Cat__‐induced increase in PGE~2~ formation was inhibited by NS‐398, a selective inhibitor of COX‐2 enzymatic activity. Ad__Cat__ infection did not change the expression of the constitutive (type 1) COX protein. Although Ad__Cu/ZnSOD__ a

Who reads Induction of Cyclooxygenase‐2 by Overexpression of the Human Catalase Gene in Cerebral Microvascular Endothelial Cells?

Author

Xiang Fang; A Steven Moore; O Joseph Nwankwo; L Neal Weintraub; W Larry Oberley; D Gary Snyder; A Arthur Spector

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)