About this Biochemistry, Genetics and Molecular Biology article

Antifungal activity of Coriaria nepalensis essential oil by disrupting ergosterol biosynthesis and membrane integrity against Candida by Aijaz Ahmad; Amber Khan; Pankaj Kumar; R. P. Bhatt; Nikhat Manzoor is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract Fungal diseases in humans have increased significantly with the advent of an expanding population of immunosuppressed patients and with the introduction of sophisticated life‐saving medical procedures. Plant extracts and products have been used in traditional medicine for centuries. __Coriaria nepalensis__ essential oil (CNEO) is known to possess antimicrobial activity. This study was an attempt to examine CNEO against various fluconazole‐sensitive and ‐resistant __Candida__ isolates. Insight into the mechanism of action was elucidated by flow cytometric analysis and ergosterol biosynthesis studies. The susceptibility tests for CNEO were carried out in terms of MIC and by disc diffusion assays against all __Candida__ isolates, employing standard protocols. Insight into the mechanism of action was elucidated by propidium iodide cell sorting (FACS) and by assessing ergosterol content in treated and untreated isolates with the test entity. CNEO was found effective against all __Candida__ isolates, including the resistant strains. While CNEO inflicts fungal cell death by disrupting membrane integrity, significant impairment of ergosterol biosynthesis was induced by the test

Author

Aijaz Ahmad; Amber Khan; Pankaj Kumar; R. P. Bhatt; Nikhat Manzoor

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)