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Voltammetric quantification, spectroscopic, and DFT studies on the binding of the antineoplastic drug Azacitidine with DNA by Pelin Şenel; Soykan Agar; Mine Yurtsever; Ayşegül Gölcü is a Chemistry article available to read on EtoBox.

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In this study, experimental studies were carried out to explore the action mechanism of the anti-cancer drug Azacitidine on the double-stranded DNA (dsDNA). The drug binding constant (K b ) was found to be 4.13 ± 0.23 × 10 5 M -1 using voltammetric measurements and 1.67 ± 0.24 × 10 5 M -1 using the fluorescence spectroscopy. Both values are close to the values of 2.04 ± 0.30 × 10 5 M -1 for deoxyguanosine (dGuO) and 1.23 ± 0.30 × 10 5 M -1 for deoxyadenosine (dAdo). In the displacement studies, the ethidium bromide, strong DNA intercalator, was replaced by the Azacitidine, hence caused a decrease on the fluorescence emission intensity. In thermal denaturation studies, the increase of 8.60 • C in the melting temperature upon introduction of the Azacitidine into the dsDNA solution cleary indicated intercalation binding mode of the drug. The experimental and theoretical IR spectra of Azacitidine, dsDNA and their H-bonded complex were confirmed the Azacitidine's intercalation ability to induce cytotoxicity. We also developed a method for the detection of Azacitidine at low concentrations using the differential pulse voltammetry (DPV). The peak current decreases in the oxidation signals

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Author

Pelin Şenel; Soykan Agar; Mine Yurtsever; Ayşegül Gölcü

Publisher

Elsevier BV

Published

2024

Language

EN

Field

Chemistry (Physical Sciences)