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Distinct roles of SOX2, Pax6 and Maf transcription factors in the regulation of lens‐specific δ 1‐crystallin enhancer by Mayumi Muta; Yusuke Kamachi; Aki Yoshimoto; Yujiro Higashi; Hisato Kondoh is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract **Background:** The eye lens provides a good model for the study of regulation of cell differentiation, in which lens‐specific δ__1‐crystallin__ expression serves as an indicator of the differentiated state of the cells. It has been indicated that the SOX2, Pax6 and Maf proteins are the major regulators of lens cell differentiation. To clarify the individual roles of these transcription factors, we analysed their participation in regulation of the δ__1‐crystallin__ enhancer. **Results:** We defined the major binding sites of SOX2, Pax6 and Maf transcription factors in the δ__1‐crystallin__ enhancer and assessed the effect of mutations at these sites in the cultured lens epithelial cells and in developing lenses of transgenic mouse embryos. SOX2 (or SOX1/SOX3) is essential for activation of the enhancer under all conditions. Pax6 bound at the δEF3 site is required for activation of the enhancer, while Pax6 at the Pax6U site appears to be involved in the Pax6‐dependent suppression of the enhancer. In contrast, Maf proteins are only required for high enhancer activity in lens fibre cells. **Conclusion:** The distinct roles of these transcription factors in the regulation o

Who reads Distinct roles of SOX2, Pax6 and Maf transcription factors in the regulation of lens‐specific δ 1‐crystallin enhancer?

Author

Mayumi Muta; Yusuke Kamachi; Aki Yoshimoto; Yujiro Higashi; Hisato Kondoh

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1356-9597)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)