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A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa by Heidi L Williams; Lynne Turnbull; Susan J Thomas; Anna Murphy; Tim Stinear; David S Armstrong; Cynthia B Whitchurch is a Medicine article available to read on EtoBox.

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## Abstract ## Background Chronic lung infection with the bacterium __Pseudomonas aeruginosa__ is one of the hallmarks of cystic fibrosis (CF) and is associated with worsening lung function, increased hospitalisation and reduced life expectancy. A virulent clonal strain of __P. aeruginosa__ (Australian epidemic strain I; AES-I) has been found to be widespread in CF patients in eastern Australia. ## Methods Suppression subtractive hybridization (SSH) was employed to identify genetic sequences that are present in the AES-I strain but absent from the sequenced reference strain PAO1. We used PCR to evaluate the distribution of several of the AES-I loci amongst a collection of 188 __P. aeruginosa__ isolates which was comprised of 35 AES-I isolates (as determined by PFGE), 78 non-AES-I CF isolates including other epidemic CF strains as well as 69 __P. aeruginosa__ isolates from other clinical and environmental sources. ## Results We have identified a unique AES-I genetic locus that is present in all 35 AES-I isolates tested and not present in any of the other 153 __P. aeruginosa__ strains examined. We have used this unique AES-I locus to develop a diagnostic PCR and a real-time PCR assay

Who reads A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa?

Author

Heidi L Williams; Lynne Turnbull; Susan J Thomas; Anna Murphy; Tim Stinear; David S Armstrong; Cynthia B Whitchurch

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2002]-; Springer Science and Business Media LLC; Springer Nature (ISSN 1476-0711)

Published

2010

Language

EN

Field

Medicine (Health Sciences)