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Can I read Systemic Immunity Upon Local Oncolytic Virotherapy Armed with Immunostimulatory Genes May Be Supported by Tumor-derived Exosomes on EtoBox?
Systemic Immunity Upon Local Oncolytic Virotherapy Armed with Immunostimulatory Genes May Be Supported by Tumor-derived Exosomes by Alireza Labani-Motlagh; Sedigheh Naseri; Jessica Wenthe; Emma Eriksson; Angelica Loskog is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Systemic Immunity Upon Local Oncolytic Virotherapy Armed with Immunostimulatory Genes May Be Supported by Tumor-derived Exosomes about?
Immunostimulatory gene therapy utilizing oncolytic viruses (OVs) as gene vehicles is a promising immunotherapy for cancer. Since viruses are immunogenic, systemic delivery can be troublesome due to neutralizing antibodies. Nevertheless, local delivery by intratumoral injection seems to induce systemic immune reactions. In this study, we demonstrate a novel mechanism of action of armed OV therapy suggesting that exosomes released by tumor cells infected with armed OV may participate to activate the immune system and this may also support systemic immunity. Tumor cell-derived exosomes commonly exert immunosuppressive functions. We hypothesized that exosomes derived from OV-infected tumor cells may instead be immunostimulatory. Human melanoma cells were infected by OVs armed with costimulatory molecules CD40 ligand (CD40L) and 4-1BB ligand (4-1BBL). Exosomes were purified and investigated for the presence of CD40L/4-1BBL mRNA and protein, and for their capacity to stimulate immune responses. The results show that the exosomes cargo transgenes. The exosomes from CD40L/4-1BBL-expressing tumor cells, or the viruses themselves, could stimulate robust dendritic cell (DC) activation with an
Who reads Systemic Immunity Upon Local Oncolytic Virotherapy Armed with Immunostimulatory Genes May Be Supported by Tumor-derived Exosomes?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Alireza Labani-Motlagh; Sedigheh Naseri; Jessica Wenthe; Emma Eriksson; Angelica Loskog
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)