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Development of Asymmetrical Flow Field-flow Fractionation–multi Angle Laser Light Scattering Analysis for Molecular Mass Characterization of Cationic Potato Amylopectin by Seungho Lee; Per-Ola Nilsson; Gunilla S Nilsson; Karl-Gustav Wahlund is a Chemistry article available to read on EtoBox.
What is Development of Asymmetrical Flow Field-flow Fractionation–multi Angle Laser Light Scattering Analysis for Molecular Mass Characterization of Cationic Potato Amylopectin about?
The goal of this study is to investigate the applicability of asymmetrical flow field-flow fractionation (AsFlFFF)-multi angle laser light scattering (MALLS), and to develop a method for analysis of cationic potato amylopectin (CPAP) having ultrahigh molecular mass (UHM ). Use of the aqueous carrier having low salt content (3 mM NaN ) resulted in a distortion r 3 in AsFlFFF fractograms of CPAP with a general pattern of a sharp rise at the beginning of the elution followed by a long tailing, probably due to combination of attractive and repulsive charge interactions (attractive interaction between CPAP molecules and the channel membrane, and repulsion among cationic CPAP molecules). As the cross flow-rate (F ) increases, c the tailing tends to increase, and the repeatability of the AsFlFFF retention data tends to decrease, which is an indication of the presence of the charge interactions. The tailing gradually decreased, and the repeatability of the AsFlFFF retention data increased, as the salt content of the carrier increased. The distortion of the fractogram finally disappeared at F of about 0.2 c ml / min and the channel flow-rate (F ) of about 1 ml / min with the aqueous carrier
Who reads Development of Asymmetrical Flow Field-flow Fractionation–multi Angle Laser Light Scattering Analysis for Molecular Mass Characterization of Cationic Potato Amylopectin?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Seungho Lee; Per-Ola Nilsson; Gunilla S Nilsson; Karl-Gustav Wahlund
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2003
- Language
- EN
- Field
- Chemistry (Physical Sciences)
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