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Effect of albumin on ^14^C‐α‐Methyl‐d‐Glucopyranoside uptake in primary cultured renal proximal tubule cells: Involvement of PLC, MAPK, and NF‐κB by Ho Jae Han; Young Joon Oh; Yun Jung Lee is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A growing body of evidence implicates albumin has an important regulatory function in renal proximal tubule cells (PTCs). In present study, the effect of bovine serum albumin (BSA) on ^14^C‐α‐methyl‐d‐glucopyranoside (α‐MG) uptake and its related signal molecules were examined in the primary cultured rabbit renal PTCs. BSA significantly increased uptake of α‐MG, a distinctive proximal tubule marker, as well as expression level of Na^+^/glucose cotransporters (SGLT1 and SGLT2) proteins. The BSA‐induced increase of α‐MG uptake was completely blocked by actinomycin D and cycloheximide. Neomycin or U 73122 (PLC inhibitors), BAPTA/AM or TMB‐8 (intracellular Ca^2+^ mobilization inhibitors) completely abolished BSA‐induced increase of α‐MG uptake. BSA significantly increased IP~s~ accumulation, but did not affect Ca^2+^ uptake. Effect of BSA on α‐MG uptake was blocked by PD 98059, but did not SB 203580. BSA increased phosphorylation of p44/42 mitogen activated protein kinase (MAPK) in a time‐dependent manner. NAC or catalase (antioxidants) significantly blocked BSA‐induced increase of H~2~O~2~ formation and α‐MG uptake. BSA activated NF‐κB translocation into nucleus. PDTC, SN5

Who reads Effect of albumin on ^14^C‐α‐Methyl‐d‐Glucopyranoside uptake in primary cultured renal proximal tubule cells: Involvement of PLC, MAPK, and NF‐κB?

Author

Ho Jae Han; Young Joon Oh; Yun Jung Lee

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)