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High‐throughput Screening of Optimal Solution Conditions for Structural Biological Studies by Fluorescence Correlation Spectroscopy by Toshihiko Sugiki; Chie Yoshiura; Yutaka Kofuku; Takumi Ueda; Ichio Shimada; Hideo Takahashi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Protein aggregation is an essential molecular event in a wide variety of biological situations, and is a causal factor in several degenerative diseases. The aggregation of proteins also frequently hampers structural biological analyses, such as solution NMR studies. Therefore, precise detection and characterization of protein aggregation are of crucial importance for various research fields. In this study, we demonstrate that fluorescence correlation spectroscopy (FCS) using a single‐molecule fluorescence detection system enables the detection of otherwise invisible aggregation of proteins at higher protein concentrations, which are suitable for structural biological experiments, and consumes relatively small amounts of protein over a short measurement time. Furthermore, utilizing FCS, we established a method for high‐throughput screening of protein aggregation and optimal solution conditions for structural biological experiments.

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Author

Toshihiko Sugiki; Chie Yoshiura; Yutaka Kofuku; Takumi Ueda; Ichio Shimada; Hideo Takahashi

Publisher

Cold Spring Harbor Laboratory Press; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0961-8368)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)