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Electrophysiologically “complex” glial cells freshly isolated from the hippocampus are immunopositive for the chondroitin sulfate proteoglycan NG2 by Gary P. Schools; Min Zhou; Harold K. Kimelberg is a Neuroscience article available to read on EtoBox.

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## Abstract We have recently described a subgroup of isolated glial fibrillary acidic protein‐positive (GFAP^+^) hippocampal astrocytes that predominantly express outwardly rectifying currents (which we term “ORAs” for outwardly rectifying astrocytes), which are similar to the currents already described for hippocampal GFAP^−^ “complex glia.” We now report that post‐recording staining of cells that were first selected as “complex” by morphology and then confirmed by their electrophysiological characteristics were NG2^+^ ∼90% of the time. Also, the morphology of freshly isolated NG2^+^ cells differs from that of isolated GFAP^+^ ORAs in having a smaller and round cell body with thinner processes, which usually are collapsed back onto the soma. Upon detailed examination, NG2^+^ cells were found to differ quantitatively in some electrophysiological characteristics from GFAP^+^ ORAs. The outward, transient K^+^ currents (__I__K~a~) in the NG2^+^ cells showed a slower decay than the __I__K~a~ in ORAs, and their density decreased in NG2^+^ cells from older animals. The other two major cation currents, the voltage‐activated Na^+^ and outwardly delayed rectifier K^+^ currents, were similar

Who reads Electrophysiologically “complex” glial cells freshly isolated from the hippocampus are immunopositive for the chondroitin sulfate proteoglycan NG2?

Author

Gary P. Schools; Min Zhou; Harold K. Kimelberg

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)

Published

2003

Language

EN

Field

Neuroscience (Life Sciences)