Can I read Selective Cytokine Gene Expression in Renal Cell Carcinoma Tumor Cells and Tumor‐infiltrating Lymphocytes on EtoBox?

Selective Cytokine Gene Expression in Renal Cell Carcinoma Tumor Cells and Tumor‐infiltrating Lymphocytes by Qiu Wang; Christine Redovan; Raymond Tubbs; Thomas Olencki; Eric Klein; Seiji Kudoh; James Finke; Ronald M. Bukowski is a Medicine article available to read on EtoBox.

What is Selective Cytokine Gene Expression in Renal Cell Carcinoma Tumor Cells and Tumor‐infiltrating Lymphocytes about?

## Abstract The progression of tumors such as renal cell carcinoma (RCC), despite the presence of substantial lymphocytic infiltrates (TIL), suggests that the ability of the local immune response to control tumor growth is impaired. Cytokine gene expression was examined to further investigate the nature of this response. Initial studies were performed with frozen tumors using PCR‐assisted mRNA amplification with cytokine‐specific primers for interleukin 10 (IL‐10), interleukin 2 (IL‐2) and interferon‐gamma (IFN‐γ). IL‐2 mRNA was not detected, despite the presence of T cells as defined by the expression of CD3γ mRNA. In contrast, mRNA for IFN‐γ was expressed in 4/9 and for IL‐10 in 5/9 tumors. To confirm this, 5 fresh tumor specimens were examined, and PCR demonstrated that IL‐10 mRNA was detectable in 4/5 tumors from which RNA was isolated at the time of nephrectomy. In these experiments multiple cycles and dilutions were employed to semi‐quantitate the expression of IL‐10. To identify potential sources of this cytokine in the tumor bed, IL‐10 mRNA expression in freshly isolated lymphocytes and tumor cells, TIL lines, cultured RCC and established RCC lines was examined. Our studies

Who reads Selective Cytokine Gene Expression in Renal Cell Carcinoma Tumor Cells and Tumor‐infiltrating Lymphocytes?

Author

Qiu Wang; Christine Redovan; Raymond Tubbs; Thomas Olencki; Eric Klein; Seiji Kudoh; James Finke; Ronald M. Bukowski

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1995

Language

EN

Field

Medicine (Health Sciences)