Can I read Role of Ca^2+^/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions: Comparison with the role of phosphotidylinositol‐3 kinase on EtoBox?

Role of Ca^2+^/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions: Comparison with the role of phosphotidylinositol‐3 kinase by Sandra Verploegen; Caroline M. van Leeuwen; Hanneke W. M. van Deutekom; Jan-Willem J. Lammers; Leo Koenderman; Paul J. Coffer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Role of Ca^2+^/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions: Comparison with the role of phosphotidylinositol‐3 kinase about?

In human neutrophils, both changes in intracellular Ca^2+^ concentrations, [Ca^2+^]~i~, and activation of phosphatidylinositol‐3 kinase (PtdIns3K) have been proposed to play a role in regulating cellular function induced by chemoattractants. In this study we have investigated the role of [Ca^2+^]~i~ and its effector molecule calmodulin in human neutrophils. Increased [Ca^2+^]~i~ alone was sufficient to induce phosphorylation of extracellular signal‐regulated protein kinase 2 (ERK2), p38 mitogen activated kinase (p38 MAPK), protein kinase B (PKB) and glycogen synthase kinase‐3α (GSK‐3α). Inhibition of calmodulin using a calmodulin antagonist __N__‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalenesulfonamide (W7), did not effect __N__‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP) induced ERK, p38 MAPK or GSK‐3α phosphorylation, but attenuated fMLP induced PKB phosphorylation. PCR analysis of human neutrophil cDNA demonstrated variable expression of members of the Ca^2+^/calmodulin‐dependent kinase family. The roles of calmodulin and PtdIns3K in regulating neutrophil effector functions were further compared. Neutrophil migration was abrogated by inhibition of calmodulin, while no effect was observ

Who reads Role of Ca^2+^/calmodulin regulated signaling pathways in chemoattractant induced neutrophil effector functions: Comparison with the role of phosphotidylinositol‐3 kinase?

Author

Sandra Verploegen; Caroline M. van Leeuwen; Hanneke W. M. van Deutekom; Jan-Willem J. Lammers; Leo Koenderman; Paul J. Coffer

Publisher

John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)

Published

2002

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)