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Wheat proteomics: Relationship between fine chromosome deletion and protein expression by Nazrul Islam; Hisashi Tsujimoto; Hisashi Hirano is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract To explore the relationship between fine chromosome deletion and protein expression in common wheat, the changes in protein composition of wheat seed proteome were investigated by using chromosome 1B. A momosomic alien chromosome addition line of common wheat was used to produce the fine deletion lines. Endosperm and embryo proteins were separated by two‐dimensional gel electrophoresis (2‐DE) and visualized by staining with Commassie Brilliant Blue, and gel images were analyzed with a computer assisted image analyzer. For the first time, fine gene locations of a few endosperm and embryo proteins were identified on the chromosome 1B. These proteins with their specific gene location on the chromosome can be used as protein markers in breeding programs for quality of wheat proteins. To identify wheat seed proteins and to understand their expression in relation to chromosome deletion, the feasibility of a new analytical approach based on isotope coded affinity tag labeling (ICAT) of peptides in tryptic digests followed by electrospray ionization mass spectrometry has been described. Simplification of the complex tryptic digest prior to mass spectral analysis was performed b

Who reads Wheat proteomics: Relationship between fine chromosome deletion and protein expression?

Author

Nazrul Islam; Hisashi Tsujimoto; Hisashi Hirano

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)