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Development of uncoated near-spherical gold nanoparticles for the label-free quantification of Lactobacillus rhamnosus GG by surface-enhanced Raman spectroscopy by Elie Akanny; Anne Bonhommé; Carine Commun; Anne Doleans-Jordheim; François Bessueille; Sandrine Bourgeois; Claire Bordes is a Chemistry article available to read on EtoBox.
What is Development of uncoated near-spherical gold nanoparticles for the label-free quantification of Lactobacillus rhamnosus GG by surface-enhanced Raman spectroscopy about?
The Surface-enhanced Raman spectroscopy (SERS) method based on gold nanoparticles as SERS substrate was investigated for the label-free detection and quantification of probiotic bacteria that are widely used in various pharmaceutical formulations. Indeed, the development of a simple and fast SERS method dedicated to the quantification of bacteria should be very useful for the characterization of such formulations in a more convenient way than the usually performed tedious and time-consuming conventional counting method. For this purpose, uncoated near-spherical gold nanoparticles were developed at room temperature by acidic treatment of star-like gold nanoparticle precursors. In this study, we first investigated the influence of acidic treatment conditions on both the nanoparticle physicochemical properties and SERS efficiency using Rhodamine 6G (R6G) as "model" analyte. Results highlighted that an effective R6G Raman signal enhancement was obtained by promoting chemical effect through R6G-anion interactions and by obtaining a suitable aggregation state of the nanoparticles. Depending on the nanoparticle synthesis conditions, R6G SERS signals were up to 10-10-fold greater than thos
Who reads Development of uncoated near-spherical gold nanoparticles for the label-free quantification of Lactobacillus rhamnosus GG by surface-enhanced Raman spectroscopy?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Elie Akanny; Anne Bonhommé; Carine Commun; Anne Doleans-Jordheim; François Bessueille; Sandrine Bourgeois; Claire Bordes
- Publisher
- Springer Science and Business Media LLC
- Published
- 2019
- Language
- EN
- Field
- Chemistry (Physical Sciences)