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Engineering Human Cell-based, Functionally Integrated Osteochondral Grafts by Biological Bonding of Engineered Cartilage Tissues to Bony Scaffolds by Celeste Scotti; Dieter Wirz; Francine Wolf; Dirk J. Schaefer; Vivienne Bürgin; Alma U. Daniels; Victor Valderrabano; Christian Candrian; Marcel Jakob; Ivan Martin; Andrea Barbero is a Engineering article available to read on EtoBox.
What is Engineering Human Cell-based, Functionally Integrated Osteochondral Grafts by Biological Bonding of Engineered Cartilage Tissues to Bony Scaffolds about?
In this study, we aimed at developing and validating a technique for the engineering of osteochondral grafts based on the biological bonding of a chondral layer with a bony scaffold by cell-laid extracellular matrix. Osteochondral composites were generated by combining collagen-based matrices (Chondro-Gide Ò ) containing human chondrocytes with devitalized spongiosa cylinders (Tutobone Ò ) using a fibrin gel (Tisseel Ò ). We demonstrate that separate pre-culture of the chondral layer for 3 days prior to the generation of the composite allows for (i) more efficient cartilaginous matrix accumulation than no pre-culture, as assessed histologically and biochemically, and (ii) superior biological bonding to the bony scaffold than 14 days of pre-culture, as assessed using a peel-off mechanical test, developed to measure integration of bilayered materials. The presence of the bony scaffold induced an upregulation in the infiltrated cells of the osteoblast-related gene bone sialoprotein, indicative of the establishment of a gradient of cell phenotypes, but did not affect per se the quality of the cartilaginous matrix in the chondral layer. The described strategy to generate osteochondral p
Who reads Engineering Human Cell-based, Functionally Integrated Osteochondral Grafts by Biological Bonding of Engineered Cartilage Tissues to Bony Scaffolds?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Celeste Scotti; Dieter Wirz; Francine Wolf; Dirk J. Schaefer; Vivienne Bürgin; Alma U. Daniels; Victor Valderrabano; Christian Candrian; Marcel Jakob; Ivan Martin; Andrea Barbero
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)
- Published
- 2010
- Language
- EN
- Field
- Engineering (Physical Sciences)