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Evolution of carrying capacity in evolution experiments focusing on a single locus on the Escherichia coli chromosome by Kotaro Mori; Akiko Kashiwagi; Itaru Urabe; Tetsuya Yomo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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We performed a series of evolution experiments, the results of which illustrated the relationship between mutations and increased carrying capacity (K). Performing an evolution experiment with repeated cycles of mutation by PCR and selection makes it possible to obtain results over shorter culture durations than in methods reported previously relying on spontaneous mutation and selection. We constructed random mutant populations of Escherichia coli in which members differed only in part of the genomic copy of the glutamine synthetase gene and performed daily serial transfer culture where the populations were in K-selected environments. The value of K in this system was increased by 10 5 -to 10 8 -fold relative to the parent clone, which was achieved by four randomly introduced mutations. This method can be applied to any gene and will be useful for analyzing a number of important issues in evolutionary biology.

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Author

Kotaro Mori; Akiko Kashiwagi; Itaru Urabe; Tetsuya Yomo

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0303-2647)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)