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Diagnosis of Tuberculosis in Children Using a Polymerase Chain Reaction by David Gomez-Pastrana; Rafael Torronteras; Pilar Caro; Maria Luisa Anguita; Antonio María López Barrio; Anselmo Andrés; Juan Navarro is a Medicine article available to read on EtoBox.

What is Diagnosis of Tuberculosis in Children Using a Polymerase Chain Reaction about?

We investigated the value of the polymerase chain reaction (PCR) in the diagnosis of active tuberculosis in children and evaluated the relationship between PCR results in children with tuberculous infections and mediastinal adenopathies detected by computerized tomography (CT-Scan). This was a controlled, blinded, prospective study comparing nested PCR, mycobacterial cultures and the clinical diagnosis based on 350 clinical specimens from 117 children referred for evaluation of suspected pulmonary tuberculosis. All children with tuberculous infection but without active disease underwent a chest CT-scan to detect the presence of mediastinal adenopathies not evident on chest x-ray. The sensitivity of PCR was 56.8% in children with clinically active disease (culture: 37.8%; smears: 13.5%). A major advantage of PCR over cultures was noted when there was no parenchymal involvement on chest radiograph and when the patient was undergoing antituberculous treatment. There were nine specimens with false-negative PCR results due to the presence of amplification reaction inhibitors. PCR was positive in five children with tuberculous infection without active disease and these children presented

Who reads Diagnosis of Tuberculosis in Children Using a Polymerase Chain Reaction?

Author

David Gomez-Pastrana; Rafael Torronteras; Pilar Caro; Maria Luisa Anguita; Antonio María López Barrio; Anselmo Andrés; Juan Navarro

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 8755-6863)

Published

1999

Language

EN

Field

Medicine (Health Sciences)