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Self-assembly and structural characterization of Echinococcus granulosus antigen B recombinant subunit oligomers by Karina M. Monteiro; Sandra M.N. Scapin; Marcos V.A.S. Navarro; Nilson I.T. Zanchin; Mateus B. Cardoso; Nádya P. da Silveira; Paulo F.B. Gonçalves; Hubert K. Stassen; Arnaldo Zaha; Henrique B. Ferreira is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Self-assembly and structural characterization of Echinococcus granulosus antigen B recombinant subunit oligomers about?

Echinococcus granulosus antigen B is an oligomeric protein of 120-160 kDa composed by 8-kDa (AgB8) subunits. Here, we demonstrated that the AgB8 recombinant subunits AgB8/1, AgB8/2 and AgB8/3 are able to self-associate into high order homo-oligomers, showing similar properties to that of parasite-produced AgB, making them valuable tools to study AgB structure. Dynamic light scattering, size exclusion chromatography and cross-linking assays revealed ~120-to 160-kDa recombinant oligomers, with a tendency to form populations with different aggregation states. Recombinant oligomers showed helical circular dichroism spectra and thermostability similar to those of purified AgB. Crosslinking and limited proteolysis experiments indicated different degrees of stability and compactness between the recombinant oligomers, with the AgB8/3 one showing a more stable and compact structure. We have also built AgB8 subunit structural models in order to predict the surfaces possibly involved in electrostatic and hydrophobic interactions during oligomerization.

Who reads Self-assembly and structural characterization of Echinococcus granulosus antigen B recombinant subunit oligomers?

Author

Karina M. Monteiro; Sandra M.N. Scapin; Marcos V.A.S. Navarro; Nilson I.T. Zanchin; Mateus B. Cardoso; Nádya P. da Silveira; Paulo F.B. Gonçalves; Hubert K. Stassen; Arnaldo Zaha; Henrique B. Ferreira

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1570-9639)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)