About this Medicine article

Pharmacological postconditioning protects against hepatic ischemia/reperfusion injury: Pharmacological Postconditioning of the Liver by Caterina Dal Ponte; Elisa Alchera; Antonia Follenzi; Chiara Imarisio; Maria Prat; Emanuele Albano; Rita Carini is a Medicine article available to read on EtoBox.

Postconditioning is a procedure based on the induction of intracellular protective reactions immediately after the onset of reperfusion. Because of the growing need to prevent ischemia/reperfusion (I/R) injury during liver surgery and transplantation, we investigated the possibility of pharmacologically inducing hepatic postconditioning. The effects of the adenosine A2A receptor agonist 2p-(2-carboxyethyl)-phenyl-amino-5 0 -N-ethylcarboxyamido-adenosine (CGS21680; 5 lmol/L) and the phosphatase and tensin homologue deleted from chromosome 10 (PTEN) inhibitor dipotassium bisperoxo-(5-hydroxypyridine-2-carboxyl)-oxovanadate [bpV(HOpic); 250 nmol/L] were investigated in primary rat hepatocytes during reoxygenation after 24 hours of cold storage and in an in vivo model of rat liver warm I/R. The addition of CGS21680 at reoxygenation significantly reduced hepatocyte death through the activation of the phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB)/ Akt signal pathway and through the reduction of the intracellular level of PTEN. PTEN lowering was associated with the increased generation of reactive oxygen species after A2A receptor-mediated stimulation of b-nicotinamide adenine d

Author

Caterina Dal Ponte; Elisa Alchera; Antonia Follenzi; Chiara Imarisio; Maria Prat; Emanuele Albano; Rita Carini

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

2011

Language

EN

Field

Medicine (Health Sciences)