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This document provides information on reagents and procedures for quantitatively determining total and direct bilirubin levels in serum or plasma using a manual or automated method. The method is based on a colorimetric reaction between bilirubin and diazotized sulphanilic acid that produces a blue complex. Total bilirubin is measured in the presence of caffeine to release albumin-bound bilirubin. Direct bilirubin is measured in the absence of caffeine. The document lists reagents, materials, clinical signi

Author

John Senufa

Language

EN