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Comparison of the proteome of Methylobacterium extorquens AM1 grown under methylotrophic and nonmethylotrophic conditions by Markus Laukel; Michel Rossignol; Gisèle Borderies; Uwe Völker; Julia A. Vorholt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract __Methylobacterium extorquens__ AM1 is a facultative methylotrophic bacterium that is capable of growing in the presence of methanol as the sole carbon and energy source, but is also able to grow on a limited number of C~2~, C~3~, and C~4~ compounds, for example succinate. This study provides a proteomic view of the cellular adaptation of __M. extorquens__ AM1 to growth on methanol and succinate, respectively. Cytosolic proteins were separated by two‐dimensional gel electrophoresis employing overlapping pH ranges and visualized by silver nitrate or fluorescence staining. A proteomic reference map containing 229 different proteins identified by peptide mass fingerprinting of tryptic fragments was established. Comparative proteome profiling of methanol‐ and succinate‐grown cells led to the identification of 68 proteins that are induced under methylotrophic growth conditions in comparison to growth on succinate. This group includes most proteins known to be directly involved in methanol oxidation to CO~2~ and in assimilation of one carbon units by the serine cycle as well as 18 proteins without any assigned function and two proteins with a predicted regulatory function. Fu

Who reads Comparison of the proteome of Methylobacterium extorquens AM1 grown under methylotrophic and nonmethylotrophic conditions?

Author

Markus Laukel; Michel Rossignol; Gisèle Borderies; Uwe Völker; Julia A. Vorholt

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)