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Regulated Targeting of BAX to Mitochondria by Ing Swie Goping; Atan Gross; Josée N. Lavoie; Mai Nguyen; Ronald Jemmerson; Kevin Roth; Stanley J. Korsmeyer; Gordon C. Shore is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Regulated Targeting of BAX to Mitochondria about?

The proapoptotic protein BAX contains a single predicted transmembrane domain at its COOH terminus. In unstimulated cells, BAX is located in the cytosol and in peripheral association with intracellular membranes including mitochondria, but inserts into mitochondrial membranes after a death signal. This failure to insert into mitochondrial membrane in the absence of a death signal correlates with repression of the transmembrane signal-anchor function of BAX by the NH2-terminal domain. Targeting can be instated by deleting the domain or by replacing the BAX transmembrane segment with that of BCL-2. In stimulated cells, the contribution of the NH2 terminus of BAX correlates with further exposure of this domain after membrane insertion of the protein. The peptidyl caspase inhibitor zVAD-fmk partly blocks the stimulated mitochondrial membrane insertion of BAX in vivo, which is consistent with the ability of apoptotic cell extracts to support mitochondrial targeting of BAX in vitro, dependent on activation of caspase(s). Taken together, our results suggest that regulated targeting of BAX to mitochondria in response to a death signal is mediated by discrete domains within the BAX polypept

Who reads Regulated Targeting of BAX to Mitochondria?

Author

Ing Swie Goping; Atan Gross; Josée N. Lavoie; Mai Nguyen; Ronald Jemmerson; Kevin Roth; Stanley J. Korsmeyer; Gordon C. Shore

Publisher

Rockefeller University Press

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)