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Presynaptic protein interactions in vivo : evidence from botulinum A, C, D and E action at frog neuromuscular junction by Dorota A. Raciborska; William S. Trimble; Milton P. Charlton is a Neuroscience article available to read on EtoBox.

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## Abstract The present study examines the paralytic action of botulinum neurotoxins at their natural target, the neuromuscular junction. We asked whether syntaxin, synaptosome‐associated protein of 25 kDa (SNAP‐25) and vesicle‐associated membrane protein (VAMP/synaptobrevin), the proteins proteolysed by botulinum, are susceptible to cleavage in frog nerve terminals, and whether they form complexes __in vivo__. In control terminals, the three SNAREs were distributed in broad bands at 1 μm intervals, at sites consistent with presynaptic Ca^2+^ channels. Within 3 h, botulinum A, C, D and E (BoNT/A/C/D/E) blocked nerve‐evoked muscle contractions but their effects on substrate immunoreactivity varied. The effect of BoNT/A on either C‐terminus or N‐terminus immunoreactivity of SNAP‐25 was undetectable after 3‐h incubation, although C‐terminus immunoreactivity was reduced after 24 h; N‐terminus immunoreactivity was not affected even after 36 h. BoNT/E reduced C‐terminus immunoreactivity of SNAP‐25 1.5 h after toxin application when transmitter release was blocked, but required 24 h to reduce N‐terminus immunoreactivity. BoNT/C reduced syntaxin immunoreactivity after 24‐h incubation but d

Who reads Presynaptic protein interactions in vivo : evidence from botulinum A, C, D and E action at frog neuromuscular junction?

Author

Dorota A. Raciborska; William S. Trimble; Milton P. Charlton

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0953-816X)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)