Opening book details…
Can I read Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary LC MALDI‐TOF/TOF MS on EtoBox?
Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary LC MALDI‐TOF/TOF MS by Emilia Hardenborg; Amelie Botling‐Taube; Jörg Hanrieder; Marit Andersson; Albert Alm; Jonas Bergquist is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary LC MALDI‐TOF/TOF MS about?
## Abstract Analysis of proteins in human body fluids is challenging since the composition of the sample often is rather complex. Here we present a method for analysis of proteins in aqueous humor from two groups of cataract patients, with and without pseudoexfoliation (PEX). Aqueous humor is an extracellular fluid contained in the anterior chamber of the eye between the cornea and iris. The limited volume of sample requires sophisticated analysis techniques. Our method is based on a total tryptic digestion of the sample followed by capillary LC‐MALDI MS and MS/MS analysis of the peptides. The method is rapid, efficient and suitable as a complement or alternative to more commonly used methods based on gel electrophoretic experiments. With this method we found and unambiguously identified 30 nonredundant proteins. Proteins found include general transport proteins such as albumin and apolipoprotein A1 but also specific proteins involved in immune response, such as complement factors. Cystatin C, clusterin, and crystallins were also found. Although the number of proteins was roughly the same in both groups there was a significant difference in their identities. These findings may give
Who reads Protein content in aqueous humor from patients with pseudoexfoliation (PEX) investigated by capillary LC MALDI‐TOF/TOF MS?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Emilia Hardenborg; Amelie Botling‐Taube; Jörg Hanrieder; Marit Andersson; Albert Alm; Jonas Bergquist
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - VCH Verlag GmbH & CO. KGaA; Wiley (ISSN 1862-8346)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)