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Simultaneous detection of HER2/neu gene amplification and protein overexpression in paraffin-embedded breast cancer by Christian Lottner; Stephan Schwarz; Simone Diermeier; Arndt Hartmann; Ruth Knuechel; Ferdinand Hofstaedter; Gero Brockhoff is a Medicine article available to read on EtoBox.
What is Simultaneous detection of HER2/neu gene amplification and protein overexpression in paraffin-embedded breast cancer about?
## Abstract The determination of HER2/neu status in breast carcinomas has become essential for the selection of breast cancer patients for Herceptin therapy. Herceptin treatment is used in patients with metastatic breast carcinoma with HER2/neu protein overexpression detected by immunohistochemistry (IHC) or gene amplification analysed by fluorescence __in situ__ hybridization (FISH). A multiparametric fluorescent approach based on the simultaneous detection of __HER2/neu__ gene amplification and protein expression was established to increase the accuracy, and to improve the reproducibility, of HER2/neu diagnostics. Based on four paraffin‐embedded breast cancer cell lines, a combined fluorescent immunostaining (FIHC) and FISH method was developed by using the PathVysion HER2 DNA Probe Kit (VYSIS) and the polyclonal antibody from the HercepTest (DAKO). Diagnostic applicability was documented on 215 formalin‐fixed primary breast carcinomas. Criteria for immunofluorescence quantification were chosen by analogy with the FDA‐approved HercepTest scoring, ranging from 0 to 3+. There was 97.7% concordance between conventional IHC and fluorescence IHC. The FISH data resulting from the multi
Who reads Simultaneous detection of HER2/neu gene amplification and protein overexpression in paraffin-embedded breast cancer?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Christian Lottner; Stephan Schwarz; Simone Diermeier; Arndt Hartmann; Ruth Knuechel; Ferdinand Hofstaedter; Gero Brockhoff
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)
- Published
- 2005
- Language
- EN
- Field
- Medicine (Health Sciences)